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User manual

Carryover

Run this study

Section titled “Run this study”
The Set up stage of the example study Creatine kinase · sample carryover in LabRuns: the study’s settings and acceptance limits.
The Set up stage of the example study Creatine kinase · sample carryover in LabRuns: the study’s settings and acceptance limits.
Figure 1. Set up for Creatine kinase · sample carryover.
The Data stage of the example study Creatine kinase · sample carryover in LabRuns: the example results as entered.
The Data stage of the example study Creatine kinase · sample carryover in LabRuns: the example results as entered.
Figure 2. Data for Creatine kinase · sample carryover.
The Results stage of the example study Creatine kinase · sample carryover in LabRuns: the outcome of the calculation and the first of its tables and figures.
The Results stage of the example study Creatine kinase · sample carryover in LabRuns: the outcome of the calculation and the first of its tables and figures.
Figure 3. Results for Creatine kinase · sample carryover.
The Report stage of the example study Creatine kinase · sample carryover in LabRuns: page 1 of the study’s report.
The Report stage of the example study Creatine kinase · sample carryover in LabRuns: page 1 of the study’s report.
Figure 4. Report for Creatine kinase · sample carryover.
Your question

Does a high sample raise the result of a low sample measured after it?

Prepare

One very low and one very high material. Patient material is best, but control or EQA material works. Enough volume for 11 low and 10 high measurements per run.

Suggested starting plan
  • 1 uninterrupted run in the fixed order L L L H H L H H L L L L H H L H H L H H L. 21 results per run.

Measure in exactly this order, with no other samples or tests in between. Repeat an interrupted run in full under a new run ID. This follows the pSMILE collection design.

Calculation minimum

One complete 21-position run. Each extra run must also meet the limit.

  1. Decide before collecting. Create the study and fill these in on Set up:

    • The error limit: 3 × SD of the low-after-low results, a positive absolute limit, or a percentage of the Low-Low mean.
    The Set up stage of the example study Creatine kinase · sample carryover in LabRuns: the study’s settings and acceptance limits.
    The Set up stage of the example study Creatine kinase · sample carryover in LabRuns: the study’s settings and acceptance limits.
    Figure 1. Set up for Creatine kinase · sample carryover.

  2. Collect and record. Collect the results as planned above and label each one with the specimen or material, level, day, run and replicate your design tracks.

  3. Enter your results. Enter run ID, position 1–21, role, material ID and result. Enter all 21 positions as measured, without averaging. For a ready-made file, use Blank template (CSV) on Data, or download the blank 21-position template.

    The Data stage of the example study Creatine kinase · sample carryover in LabRuns: the example results as entered.
    The Data stage of the example study Creatine kinase · sample carryover in LabRuns: the example results as entered.
    Figure 2. Data for Creatine kinase · sample carryover.

  4. Calculate and check. Save the study, choose Calculate results, then check:

    • The sequence matches the run.
    • Carryover below the limit in every run. A value equal to the limit fails.
    The Results stage of the example study Creatine kinase · sample carryover in LabRuns: the outcome of the calculation and the first of its tables and figures.
    The Results stage of the example study Creatine kinase · sample carryover in LabRuns: the outcome of the calculation and the first of its tables and figures.
    Figure 3. Results for Creatine kinase · sample carryover.

  5. Report your decision. Open Report, look over the result and the study details, then download or print the report. Sign-off is optional.

    The Report stage of the example study Creatine kinase · sample carryover in LabRuns: page 1 of the study’s report.
    The Report stage of the example study Creatine kinase · sample carryover in LabRuns: page 1 of the study’s report.
    Figure 4. Report for Creatine kinase · sample carryover.

Set up

Section titled “Set up”
  1. Choose New study, select Carryover under Sample and material effects, enter Analyte and Unit, and choose Create study, or Create and import data if you already have results.
  2. On Set up, under Carryover · 21-position sequence, choose the Error-limit basis: 3 × SD of low-after-low results (the default), a user-defined limit in the study unit, or Percentage of Low-Low mean. For a user-defined limit, enter the error limit in the study unit; for a percentage, enter Error limit (% of Low-Low mean).
  3. On Data, choose Start a blank 21-position run, or Append a blank 21-position run for each further run, and type the results. Or fill in the blank template and choose Import a file; see Import a file and Columns.

When a limit is not met

Section titled “When a limit is not met”
  1. Read the reason for an undecided run. A missing or excluded position, or a run measured in another order, means repeating the run.
  2. Recheck the data of a failing run. A run that fails on good data shows carryover from the high material into the next low.

Common mistakes

Section titled “Common mistakes”
  • Using one material ID for both materials. Give the low and the high material different IDs in each run.
  • Reusing a run ID for two experiments. Give each run its own ID.
  • A blank result, or two rows in a run sharing a position. Fill in the result, and use each position from 1 to 21 once in each run.
  • A role that doesn't match the sequence. Enter low or high as the position requires; the run is otherwise Undecided.
  • A qualified result such as <5. It can't be used. Enter the exact reading from the original record; an excluded position leaves the run Undecided.
  • No error limit with a user-defined or percentage basis. Enter it on Set up before you calculate.